In order to develop antitumor agent which indicates weak side effects and strong antitumor activity, cytotoxicity and antitumor effects of methyl gallate (MG) were evaluated by MTT and SRB assay of colorimetric assay methods on the cultured NIH 3T3 fibroblasts and human oral epitheloid carcinoma cells (KB cells). KB cells were cultured in EMEM and RPMI 1640 media containing 10% fetal bovine serum, antibiotics and fungizone. After incubation for 24 hrs, the cells were treated with MG (1, 25, 50, 100 u M) for 48 hrs under the same condition.
The MTT and SRB quantity were measured by ELISA reader (Spectra Max 250, USA). The micnscopic study was carried out to observe morphological change, argyrophylic nucleolar organizer regions (Ag-NORs) number and PAS positive reaction of cultured KB cells.
The results were as follows;
1. The MTT50 were 16677 p M in NIH 3T3 cell and 65.55 p M in KB cells treated with MG.
2. The SRB50 were 8701.23,u M in NIH 3T3 cell and 168.81 p M in KB cells treated with MG.
3. The number of Ag-NORs was not significant in cultured NIH 3T3 cells, but the number of
Ag-NORs was significantly decreased in cultured KB cells treated with MG.
4. PAS reaction of cultured NIH 3T3 cells was not changed, but PAS reaction of cultured KB cells
was changed to negative reaction.
5. Morphological changes of cultured NIH 3T3 cells treated with MG were weak, but KB cell treated with MG were severe.
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